How to Build CHD@ZJU

CHD related Articles were retrieved from Pubmed, by entering keywords "coronary heart disease" and constrict the publish date from 2000/1/1 to now (2013/1/23). As a result, totally 115898 articles were found and their abstracts were downloaded for text mining. Since some articles didn't contain abstracts, only 88396 abstracts remained.

The text-mining process to get CHD related genes could be divided in to 5 following steps:

  • 1) Extracting all keywords from abstracts and ignoring those keywords start with numbers. 101402 keywords were extracted.

  • 2) Input these keywords into Gene library in ArrayTrack and find possible related genes. 4674 genes were then found.

  • 3) Put these 4674 genes again into pubmed abstracts to find related aticles. Only genes which offical name or there keyword description (such as prolactin for gene PRL) could be found in the abstract would be remained. As a result, 1247 genes were remained.

  • 4) Manually examined on the 1247 genes to validate it was acutally related to CHD. Some genes would be filtered if it represents other meanings (such as gene CAD, Entrez ID:790, carbamoyl-phosphate synthetase 2, is mostly meant coronary arterial disease in articles). 681 genes were then validated with at least one reference.

  • 5) All genes was compared with 1078 CHD genes in RGD database, and 370 genes were overlapped. These 370 genes were labels as "RGD_Supported" and the other 293 genes were labels as "REFERED". All 663 genes had supported references in CHD@ZJU which were examined by step 4.
  • How To contact Us

    Collaboration Information: Prof. Xiaohui Fan (fanxh@zju.edu.cn)

    Website using assistance : Leihong Wu (11019004@zju.edu.cn)



    Basic Information

    Gene Name: ADAM15

    Description: ADAM metallopeptidase domain 15

    Entrez Gene ID: 8751

    SwissProt Acc Number: Q13444

    RefSeq: NM_003815

    It was supported literatures to be a novel CHD related gene:

    .."These results suggest that endothelial cell-derived ADAM15, signaling through c-Src and c-Yes, contributes to atherosclerotic lesion development by disrupting adherens junction integrity and promoting monocyte transmigration."..

    From PMID: 22904271, in Journal Arterioscler Thromb Vasc Biol. , 2012


    References

    There were 1 potential papers with ADAM15 and CHD.

    PMIDTitleJournalsDetails
    20227692 ADAM33 expression in atherosclerotic lesions and relationship of ADAM33 gene variation with atherosclerosis.AtherosclerosisMore Details

    NOTEs: These result is mostly from TEXT-MINING and there might have mismatches.



    PPI interactions

    There were totally 29 unique genes interacted with ADAM15. Show PPI network

    Gene nameInteraction typereference PMIDCHD relation
    TRIP13 Two-hybrid16189514 No
    LCK Affinity Capture-Western11741929|11741929|16374509|11741929,16374509 No
    GRB2 Affinity Capture-Western11741929|11741929|16374509|11741929,16374509 CHD related
    MAD2L1 Affinity Capture-Western11741929 No
    SRC Reconstituted Complex16374509|11741929,16374509 CHD related
    SNX30 Reconstituted Complex16374509 No
    NPHP1 Reconstituted Complex16374509|16374509 No
    ARHGEF7 Reconstituted Complex16374509|16374509 No
    LYN Reconstituted Complex16374509|16374509 No
    YES1 Reconstituted Complex16374509|16374509 No
    SH3PXD2A Reconstituted Complex16374509|16374509 No
    PACSIN3 Reconstituted Complex16374509 No
    SH3GLB1 Reconstituted Complex16374509 No
    ARHGEF6 Reconstituted Complex16374509|16374509 No
    NCK1 Reconstituted Complex16374509|16374509 No
    SORBS2 Reconstituted Complex16374509|16374509 No
    SH3RF3 Reconstituted Complex16374509|16374509 No
    ABL1 in vitro;in vivo11741929 No
    SNX33 in vitro;in vivo16374509 No
    SORBS3 in vitro16374509 No
    SORBS1 in vitro16374509 No
    SH3RF1 in vitro16374509 No
    SNX9 Two-hybrid10531379|10531379,16374509 No
    MAD2L2 Two-hybrid10527948 No
    SH3GL2 Two-hybrid10531379 No
    FYN Reconstituted Complex11741929 No
    HCK Biochemical Activity11741929 No
    UBC Affinity Capture-MS21987572|21906983 No
    SH3D19 in vivo15280379 No


    Involved FDA drugs

    There was no drug associated with ADAM15