How to Build CHD@ZJU

CHD related Articles were retrieved from Pubmed, by entering keywords "coronary heart disease" and constrict the publish date from 2000/1/1 to now (2013/1/23). As a result, totally 115898 articles were found and their abstracts were downloaded for text mining. Since some articles didn't contain abstracts, only 88396 abstracts remained.

The text-mining process to get CHD related genes could be divided in to 5 following steps:

  • 1) Extracting all keywords from abstracts and ignoring those keywords start with numbers. 101402 keywords were extracted.

  • 2) Input these keywords into Gene library in ArrayTrack and find possible related genes. 4674 genes were then found.

  • 3) Put these 4674 genes again into pubmed abstracts to find related aticles. Only genes which offical name or there keyword description (such as prolactin for gene PRL) could be found in the abstract would be remained. As a result, 1247 genes were remained.

  • 4) Manually examined on the 1247 genes to validate it was acutally related to CHD. Some genes would be filtered if it represents other meanings (such as gene CAD, Entrez ID:790, carbamoyl-phosphate synthetase 2, is mostly meant coronary arterial disease in articles). 681 genes were then validated with at least one reference.

  • 5) All genes was compared with 1078 CHD genes in RGD database, and 370 genes were overlapped. These 370 genes were labels as "RGD_Supported" and the other 293 genes were labels as "REFERED". All 663 genes had supported references in CHD@ZJU which were examined by step 4.
  • How To contact Us

    Collaboration Information: Prof. Xiaohui Fan (fanxh@zju.edu.cn)

    Website using assistance : Leihong Wu (11019004@zju.edu.cn)



    Basic Information

    Gene Name: GNB3

    Description: "guanine nucleotide binding protein (G protein), beta polypeptide 3"

    Entrez Gene ID: 2784

    SwissProt Acc Number: P16520

    RefSeq: NM_002075

    It was suspected to be CHD related:

    .."Thus, in the Russian population coronary artery disease is associated with GNB3 allele C and genotype CC"..

    From PMID: 17958314, in Journal Genetika , 2007


    References

    There were 16 potential papers with GNB3 and CHD.

    PMIDTitleJournalsDetails
    23595158 Association of the G-protein beta3 subunit gene polymorphism with the incidence of cardiovascular disease independent of hypertension: the Funagata study.Journal of human hypertensionMore Details
    21742104 Polymorphisms associated with in vitro aspirin resistance are not associated with clinical outcomes in patients with coronary artery disease who report regular aspirin use.American heart journalMore Details
    20096003 G-protein beta3 subunit polymorphism and bleeding in the orbofiban in patients with unstable coronary syndromes-thrombolysis in myocardial infarction 16 trial.Journal of thrombosis and haemostasis : JTHMore Details
    20629643 "Genetic association study of the GNB3 C825T, the ACE I/D and the eNOS G894T polymorphisms and the risk to develop erectile dysfunction in a German ED population."AndrologiaMore Details
    16908025 G-protein beta3 subunit (GNB3) gene polymorphisms and cardiovascular disease: the Ludwigshafen Risk and Cardiovascular Health (LURIC) study.AtherosclerosisMore Details
    17663734 Heritability of platelet function in families with premature coronary artery disease.Journal of thrombosis and haemostasis : JTHMore Details
    17065478 Mutation in the guanine nucleotide-binding protein beta-3 causes retinal degeneration and embryonic mortality in chickens.Investigative ophthalmology & visual scienceMore Details
    15869758 Metabolic syndrome in aboriginal Canadians: prevalence and genetic associations.AtherosclerosisMore Details
    16569552 "Effects of lifestyle modification on the progression of coronary atherosclerosis, autonomic function, and angina--the role of GNB3 C825T polymorphism."American heart journalMore Details
    15917856 Association between beta-adrenergic receptor polymorphisms and their G-protein-coupled receptors with body mass index and obesity in women: a report from the NHLBI-sponsored WISE study.International journal of obesity (2005)More Details
    15699455 Hypertension genes are genetic markers for insulin sensitivity and resistance.HypertensionMore Details
    15389246 C825T polymorphism of the G-protein beta3 subunit gene and atrial fibrillation: association of the TT genotype with a reduced risk for atrial fibrillation.American heart journalMore Details
    12724620 Role of the eNOS Glu298Asp variant on the GNB3825T allele dependent determination of alpha-adrenergic coronary constriction.PharmacogeneticsMore Details
    12825146 Genetic risk factors for erectile dysfunction and genetic determinants of drug response--on the way to improve drug safety?HerzMore Details
    12439636 [Genetic factors in myocardial infarction--Results from a candidate gene and a genome-wide approach between beta blockers].HerzMore Details
    11350093 The human G-protein beta3 subunit C825T polymorphism is associated with coronary artery vasoconstriction.European heart journalMore Details

    NOTEs: These result is mostly from TEXT-MINING and there might have mismatches.



    PPI interactions

    There were totally 15 unique genes interacted with GNB3. Show PPI network

    Gene nameInteraction typereference PMIDCHD relation
    PDCL Affinity Capture-Western19376773 No
    TGFBR1 in vivo15761153 CHD related
    git11 Two-hybrid16884933 No
    GNG3 Two-hybrid8636150|19168127 No
    GNG4 Two-hybrid8636150|19168127|8636150,16884933 No
    GNG5 Two-hybrid8636150|19168127|8636150 No
    GNG7 Two-hybrid8636150|19168127|8636150 No
    GNG2 Affinity Capture-Western10339615|19168127|9789084 No
    RGS6 Affinity Capture-Western10339615 No
    GNGT2 Affinity Capture-Western19168127 No
    GNG10 Affinity Capture-Western19168127 No
    GNG12 Affinity Capture-Western19168127 No
    GNG13 Affinity Capture-Western19168127|12454992 No
    GNG11 Affinity Capture-Western19168127 No
    RASD2 Two-hybrid19255495 No


    Involved FDA drugs

    There was no drug associated with GNB3