How to Build CHD@ZJU

CHD related Articles were retrieved from Pubmed, by entering keywords "coronary heart disease" and constrict the publish date from 2000/1/1 to now (2013/1/23). As a result, totally 115898 articles were found and their abstracts were downloaded for text mining. Since some articles didn't contain abstracts, only 88396 abstracts remained.

The text-mining process to get CHD related genes could be divided in to 5 following steps:

  • 1) Extracting all keywords from abstracts and ignoring those keywords start with numbers. 101402 keywords were extracted.

  • 2) Input these keywords into Gene library in ArrayTrack and find possible related genes. 4674 genes were then found.

  • 3) Put these 4674 genes again into pubmed abstracts to find related aticles. Only genes which offical name or there keyword description (such as prolactin for gene PRL) could be found in the abstract would be remained. As a result, 1247 genes were remained.

  • 4) Manually examined on the 1247 genes to validate it was acutally related to CHD. Some genes would be filtered if it represents other meanings (such as gene CAD, Entrez ID:790, carbamoyl-phosphate synthetase 2, is mostly meant coronary arterial disease in articles). 681 genes were then validated with at least one reference.

  • 5) All genes was compared with 1078 CHD genes in RGD database, and 370 genes were overlapped. These 370 genes were labels as "RGD_Supported" and the other 293 genes were labels as "REFERED". All 663 genes had supported references in CHD@ZJU which were examined by step 4.
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    Collaboration Information: Prof. Xiaohui Fan (

    Website using assistance : Leihong Wu (

    Basic Information

    Gene Name: GRK4

    Description: G protein-coupled receptor kinase 4

    Entrez Gene ID: 2868

    SwissProt Acc Number: P32298

    RefSeq: NM_001004056

    It was supported literatures to be a novel CHD related gene:

    .."These results suggest that the presence of GRK4 variant alleles may be important determinants of blood pressure response to atenolol and risk for adverse cardiovascular events. The associations with GRK4 variant alleles were stronger in patients who were also ADRB1 389R homozygotes, suggesting a potential interaction between these 2 genes."..

    From PMID: 22949529, in Journal Hypertension , 2012


    There were 1 potential papers with GRK4 and CHD.

    22949529 G protein receptor kinase 4 polymorphisms: beta-blocker pharmacogenetics and treatment-related outcomes in hypertension.HypertensionMore Details

    NOTEs: These result is mostly from TEXT-MINING and there might have mismatches.

    PPI interactions

    There were totally 6 unique genes interacted with GRK4. Show PPI network

    Gene nameInteraction typereference PMIDCHD relation
    FSHR Biochemical Activity10379886|10379886 No
    BDKRB2 in vitro;in vivo11517230 CHD related
    CALM1 Reconstituted Complex9092566 No
    IKBKG Reconstituted Complex20098747 No
    NEDD4 Reconstituted Complex19953087 No
    UBC Affinity Capture-MS21890473 No

    Involved FDA drugs

    There was no drug associated with GRK4