How to Build CHD@ZJU

CHD related Articles were retrieved from Pubmed, by entering keywords "coronary heart disease" and constrict the publish date from 2000/1/1 to now (2013/1/23). As a result, totally 115898 articles were found and their abstracts were downloaded for text mining. Since some articles didn't contain abstracts, only 88396 abstracts remained.

The text-mining process to get CHD related genes could be divided in to 5 following steps:

  • 1) Extracting all keywords from abstracts and ignoring those keywords start with numbers. 101402 keywords were extracted.

  • 2) Input these keywords into Gene library in ArrayTrack and find possible related genes. 4674 genes were then found.

  • 3) Put these 4674 genes again into pubmed abstracts to find related aticles. Only genes which offical name or there keyword description (such as prolactin for gene PRL) could be found in the abstract would be remained. As a result, 1247 genes were remained.

  • 4) Manually examined on the 1247 genes to validate it was acutally related to CHD. Some genes would be filtered if it represents other meanings (such as gene CAD, Entrez ID:790, carbamoyl-phosphate synthetase 2, is mostly meant coronary arterial disease in articles). 681 genes were then validated with at least one reference.

  • 5) All genes was compared with 1078 CHD genes in RGD database, and 370 genes were overlapped. These 370 genes were labels as "RGD_Supported" and the other 293 genes were labels as "REFERED". All 663 genes had supported references in CHD@ZJU which were examined by step 4.
  • How To contact Us

    Collaboration Information: Prof. Xiaohui Fan (

    Website using assistance : Leihong Wu (

    Basic Information

    Gene Name: NOS1AP

    Description: nitric oxide synthase 1 (neuronal) adaptor protein

    Entrez Gene ID: 9722

    SwissProt Acc Number: O75052

    RefSeq: NM_001126060

    It was supported literatures to be a novel CHD related gene:

    ..Positive Association Between rs10918859 of the NOS1AP Gene and Coronary Heart Disease in Male Han Chinese..

    From PMID: 23171141, in Journal Genet Test Mol Biomarkers. , 2013


    There were 6 potential papers with NOS1AP and CHD.

    23228162 "Diabetes, lower extremity amputation, loss of protective sensation, and neuronal nitric oxide synthase associated protein in the chronic renal insufficiency cohort study."Wound repair and regeneration : official publication of the Wound Healing SocietyMore Details
    23171141 Positive association between rs10918859 of the NOS1AP gene and coronary heart disease in male Han Chinese.Genetic testing and molecular biomarkersMore Details
    22133205 Allelic variant of NOS1AP effects on cardiac alternans of repolarization during exercise testing.Scandinavian journal of clinical and laboratory investigationMore Details
    21685173 "Common variants in CASQ2, GPD1L, and NOS1AP are significantly associated with risk of sudden death in patients with coronary artery disease."Circulation. Cardiovascular geneticsMore Details
    21658281 GWAS for discovery and replication of genetic loci associated with sudden cardiac arrest in patients with coronary artery disease.BMC cardiovascular disordersMore Details
    19204306 Genetic variations in nitric oxide synthase 1 adaptor protein are associated with sudden cardiac death in US white community-based populations.CirculationMore Details

    NOTEs: These result is mostly from TEXT-MINING and there might have mismatches.

    PPI interactions

    There were totally 9 unique genes interacted with NOS1AP. Show PPI network

    Gene nameInteraction typereference PMIDCHD relation
    SYN1 Reconstituted Complex11867766|11867766 No
    SYN2 Reconstituted Complex11867766|11867766 No
    SYN3 Reconstituted Complex11867766|11867766 No
    APP Reconstituted Complex21832049 No
    LRP2 Two-hybrid10827173 No
    LRP1 Two-hybrid10827173 CHD related
    LRP8 Two-hybrid10827173 CHD related
    RASD1 Reconstituted Complex11086993|11086993|11086993 No
    NOS1 Two-hybrid9459447|9459447|9459447 CHD related

    Involved FDA drugs

    There was no drug associated with NOS1AP